CloneID: OX-89
Antigen Long Description: This antibody was prepared by immunizing PVG rats with a recombinant chimeric protein consisting of domains 3 and 4 of rat CD4 and the extracellular region of mouse CD134L.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P43488
Specificity Statement: This antibody is specific for murine CD252 (the ligand for OX40), expression of which is restricted to activated B cells, dendritic cells, microglia and endothelial cells.
Application Notes (Clone): This antibody binds mouse CD134L, and has been shown to bind activated B cells and DCs, as well as CD134L-transfected fibroblast cells, though not activated T cells, in labelling and immunohistochemical analysis (Malmström, 2001). In addition, this antibody is able to partially block the interaction between CD134 and CD134L. This blocking effect could be responsible for the ability of this antibody to prevent T cell accumulation in the intestine of T cell-restored SCID mice and inhibits the development of Th1-mediated colitis.
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