CloneID: KGH-R1
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with H-p21Ras was used as an immunogen.
Origin Pub PMID: 26897358
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P01116; P01112; P01111
Specificity Statement: This antibody recognizes human wild-type and mutant H-, K-, and N-Ras proteins. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic ras mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Application Notes (Clone): The binding reactivity of this antibody to H-p21Ras, K-p21Ras and N-p21Ras protein was determined using ELISA. This antibody was also capable of recognizing wild type and mutated ras protein from various normal and tumor cell lines using western blot and immunocytometry assay. This antibody presented strong immunoreactivity for 14 primary human cancer tissues but showed weak or negative immunoreactivity in the corresponding normal tissues. It was reported to bind positively with QGY7703, SMMC7721, HepG2: hepatocarcinoma; BGC853, MKN28: gastric cancer; HCT116: colorectal cancer; T24: bladder cancer; SKOV3: ovary cancer; MDA-MB-231, MDA-MB-435, MCF7: breast cancer; HeLa: cervical cancer; Hep2: laryngocarcinoma; C8166, K562 cell lines (PMID: 26897358). This antibody was also used in the immunohistochemical staining of FFPE section of colorectal tissues, colorectal inflammatory polyps, colorectal low-grade intraepithelial neoplasia, colorectal high-grade intraepithelial neoplasia, invasive colorectal carcinomas and corresponding adjacent tissues. The immunoreactivity was measured by evaluating percentage of positive cells and histological score (HSCORE) (PMID: 21138685). This antibody can also be used in the immunofluorescent staining of various normal and cancer tissues (PMID: 32664770).
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