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Application: Western blot, ELISA
Description: Purified, recombinant, chimeric murine/human anti-PD-L1 (IgG1) antibody with C-terminal Avi-tag, expressed in HEK293 cells. Purified antibody was enzymatically biotinylated using Avitag™ technology. This antibody reacts with human PD-L1 and cross-reacts with mouse PD-L1. It has not been tested for cross-reactivity with other species.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purification: Protein A affinity chromatography from HEK293 supernatants
Storage Stability: Stable for at least 12 months at -80°C.
Tags: Biotin
Uniprot: Q9NZQ7
Warnings: Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Biosafety Level: Not applicable (BSL-1)
References: 1. Freeman, G.J., et al., J. Exp. Med. 2000;192(7): 1027-1034
2. Dong. H., et al., Nat. Med. 1999; 5(12):1365-1369.
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