CloneID: F9
Antigen Long Description: To generate this antibody a synthetic human scFv library encoding HA-tagged scFv clones was used for biopanning against pep27.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q8DQS1
Specificity Statement: This antibody is specific for the pep27 protein of Streptococcus pneumoniae. Specifically the epitope Y24, R26 and K2. Pep27 is a secreted peptide which is the signal sensed by VncR/S.
Application Notes (Clone): An western blot was performed on proteins from S.aureus using the scFv version of this antibody, it showed that the antibody was specific for the pep27 protein. Furthermore an ELISA was performed on His6-MBP-Pep27, it showed specificity of this antibody to pep27 (Kim et al, 2018; pmid: 29753735).
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