CloneID: pT231/pS235_1
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents, assays, and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P10636
Specificity Statement: This antibody shows binding affinity towards pT231/pS235 peptide of the human tau protein. The binding affinity and specificity of this antibody relied heavily on the phosphorylation at Thr-231.
Application Notes (Clone): ELISA was used for the screening of positive clones that bind phosphopeptides. The binding affinity of the IgG antibody towards pT231/pS235 phosphopeptide was found to be Kd = 0.35 nM using surface plasmon resonance. This antibody can recognize the phospho-tau epitopes in full-length human tau. This antibody was also used for the immunohistochemical analysis of the human brain with Alzheimer's disease (PMID: 23148212).
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