CloneID: Nb01
Antigen Long Description: The original antibody was isolated from a nanobody phage-display naïve library generated from the peripheral blood of llamas immunized with purified periplasmic domain (residues 36–516) of PorM of Porphyromonas gingivalis. The specific antibody was isolated by panning the library against periplasmic domain of PorM protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: B2RLE8
Specificity Statement: This antibody binds N-terminal part comprising amino acids from 44–217 of the PorM protein of Porphyromonas gingivalis. PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans.
Application Notes (Clone): The binding specificity of this antibody towards N-terminal part of the PorM protein was determined using ELISA. The affinity of the antibody was found to be Kd= 1.8 ± 0.7 nM (PMID: 28471361).
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