Anti-Prion protein (VRQ14)

Anti-Prion protein [VRQ14], Recombinant, IgG kappa, Rabbit

CloneID: VRQ14

Antigen Long Description: The VRQ14 antibody was prepared by immunizing Prnp0/0 mice with the C-terminal domain of recombinant ovine VRQ variant prion protein. The Fab fragemnt was produced by digestion with papain.

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P23907

Specificity Statement: VRW14 binds to both PrPC (host prion protein) and to PrPSc (pathogenic isoform of the prion protein) - the epitope of the antibody mainly consists of PrP residues 188–199 (C-terminal part of helix H2 and N-terminal part of the H2–H3 loop). Misfolding and aggregation of mutated protein PrP leads to prion diseases which are associated with the conversion of the α-helix rich prion protein (PrPC) into a β-structure-rich insoluble conformer (PrPSc). Prion diseases are neurodegenerative pathologies which affect numerous mammalian species and different mutations in the PRNP gene result in protein products with different susceptibilities to causing disease.

Application Notes (Clone): VRQ14 can be used to facilitate the crystallisation of ovine PrP in addition to being used in (sandwich) ELISA and IP studies.

• Artikelnummer: ABAAb00752-23.0
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00752-23.0
• Verpackungseinheit: 200 μg
• Mengeneinheit: STK
• Reaktivität: Sheep (Ovine)
• Klonalität: Recombinant
• Methode: Immunoprecipitation, ELISA
• Isotyp: IgG kappa
• Wirt: Rabbit