CloneID: 3A3E1
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing mice against purified human properdin.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P27918
Specificity Statement: The antibody is specific for properdin. It recognizes human, baboon, rabbit, and canine properdin, but not mouse, rat, feline or ferret properdin.
Application Notes (Clone): The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected only non-reduced properdin as evaluated by western blot analysis. The antibody inhibited AP-mediated hemolysis of rabbit erythrocytes. The ability of the antibody to inhibit the binding of properdin to C3b was tested by flow cytometry. The antibody completely impaired the binding of properdin to C3b-coated erythrocytes (ESC3b) (Chen et al. 2020; PMID: 32793201). Further, the antibody inhibited PGA formation relative to TRAP alone (Blatt et al., 2016; PMID: 27183616).
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