CloneID: 6H10
Antigen Long Description: This antibody was raised by immunising PrP-ablated (Prnp-/-) mice with PrPSc purified from the brains of mice infected with the Obihiro prion strain.
Origin Pub PMID: 26937029
Buffer Composition: PBS only.
Uniprot Accession No.: P23907
Specificity Statement: This antibody is specific for PrPSc, and binds to a conformational epitope that incudes the extreme C-terminus of PrP. It does not react with recombinant mouse PrP or denatured PrPSc.
Application Notes (Clone): The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).
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