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Application: Useful for binding assays.
Assay Conditions: A white 96-well plate was precoated with PTK7, His-Tag protein (AcroBiosystems # PT7-H52H3) (100 ng/well). Blocking Buffer 3 (#79743) and PBST were used for blocking and washing steps, respectively. For protein titration a serial dilution of Anti-PTK7 Antibody, Biotin-Labeled (#102726) at the highest concentration of 4 ng/µl (200 ng/reaction) was prepared in 1x PP-02 Assay Buffer (#82620). Diluted Anti-PTK7 was then added to the wells and plate was incubated at Room Temperature (RT) for 1 hour. The plate was then washed again and incubated with anti-Fc-HRP conjugate diluted 1:1000 in Blocking Buffer 3 for 1 hour at RT. After the final wash, ELISA ECL mix (#79670) was added to each well and chemiluminescence was read.
Background: PTK7 (Protein Tyrosine Kinase 7) is a pseudokinase of the receptor tyrosine kinase (RTK) family and shares a common ligand (Wnt5a) with fellow pseudokinases ROR1 (receptor tyrosine kinase-like orphan receptor 1) and ROR2. Despite the lack of a functional kinase domain, PTK7 still contributes to downstream signaling of Wnt5a through its interaction with LRP6 (low-density lipoprotein receptor-related protein 6) and FZD (frizzled) receptors. PTK7 expression contributes to oncogenic potential in a variety of solid tumors including ovarian, colorectal and breast cancers and neuroblastoma. The cell surface expression and oncogenic function of PTK7 in a variety of tumor types makes it a compelling target for both CAR (chimeric antigen receptor)-T cell and antibody based therapeutic development. The use of cofetuzumab pelidotin, an ADC (antibody-drug conjugate) based on auristatin and targeting PTK7, showed promise in the treatment of ovarian cancer, NSCLC (non-small cell lung cancer) and TNBC (triple-negative breast cancer), confirming the potential of targeting PTK7 in oncology.
Description: Recombinant human anti-PTK7 antibody recognizing human PTK7 (Protein Tyrosine Kinase 7) protein. This anti-PTK7 antibody is a purified, humanized recombinant monoclonal antibody, which contains an Avi-Tag™ and is biotinylated at the C-terminus of the heavy chain unit. This antibody has been tested in a binding assay. This antibody is similar to Cofetuzumab.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host Cell Line: HEK293
Purity: ≥85%
Storage Stability: At least 6 months at -80°C.
Target: PTK7
Uniprot: Q13308
Unit Definition: Antibody
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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