CloneID: III20
Antigen Long Description: The original scFv antibody was selected from a human synthetic scFv phage display library by panning against the PV11 strain of the virus.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody binds the PV11 strain of the rabies virus.
Application Notes (Clone): The specificity of the scFv-Fc format of the antibody was confirmed by ELISA analysis. The scFv-Fc version of the antibody detected GPRV by western blot analysis. The scFv-Fc version of the antibody bound specifically to the membranes of PV11 infected Vero cells in an immunofluorescence assay. The scFv-Fc version of the antibody neutralized the PV11 strain in a standard in vivo neutralization assay where the virus was incubated with the scFv-Fc fragment before intracranial inoculation in mice (Ray et al., 2001; PMID: 11472431).
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