CloneID: 1H6
Heavy Chain modification: Fc Silent™
Antigen Long Description: Oxy-2 or d(TTTTGGGG)2.
Buffer Composition: PBS only.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody binds to quadruplex DNA with a Kd of 0.33 nM. Binds to both types of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2) and Ver-3 (d(TGGGGG(TTAGGG)2T).
Application Notes (Clone): This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats. Ab00389-1.1 was used by Hansel-Hertsch et al. 2016 (PMID: 27618450) in immunofluorescence staining (in comparison to the BG4 anti-G quadruplex antibody, c.f. Ab00174).
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