CloneID: 14F3
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing CB6 fl mice with human RANKL protein.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: O14788
Specificity Statement: The antibody is specific for human-RANK-L. It does not recognize murine RANK-L. RANKL is a tumor necrosis factor (TNF)-related protein that binds to, and activates, the TNF receptor (TNFR)-related protein Receptor Activator of NF-kB (RANK). RANKL acts as the key cytokine that regulates osteoclast differentiation and activation during normal bone remodeling and during disease
Application Notes (Clone): The specificity of the original version of the antibody was confirmed by ELISA analysis (IC50 = 2 nM). The antibody specifically inhibits the functional activity of RANKL induction of DC maturation. The antibody could strongly inhibit the interaction between human RANKL and its receptor RANK in human monocytes. The inhibition led to antagonism of RANKL-mediated DC maturation and osteoclast development and function (US20040213788).
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