Anti-Rhodopsin (Rho 1D4)

Anti-Rhodopsin [Rho 1D4], Recombinant, IgG1 kappa, Rat

CloneID: Rho 1D4

Heavy Chain modification: Fc Silent™

Antigen Long Description: Bleached bovine rod outer segment (ROS).

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original mouse IgG1 format for improved compatibility with existing reagents, assays and techniques.

Uniprot Accession No.: P08100

Specificity Statement: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Application Notes (Clone): The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

• Artikelnummer: ABAAb00337-6.4-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00337-6.4-BT
• Verpackungseinheit: 1 mg
• Mengeneinheit: STK
• Reaktivität: Human, Mouse (Murine), Rat (Rattus), Various species, Cow (Bovine), Zebrafish
• Klonalität: Recombinant
• Methode: Immunofluorescence, Immunoprecipitation, Western Blotting, ELISA, Immunohistochemistry, Radioimmunoassay (RIA)
• Isotyp: IgG1 kappa
• Wirt: Rat