Anti-Sag1 (4F11E12)

Anti-Sag1 [4F11E12], Recombinant, IgM kappa, Human

CloneID: 4F11E12

Antigen Long Description: 4F11E12 was generated by intraperitoneal infection of mice with cysts from the 76 K T. gondii strain and was selected for its ability to inhibit efficiently the recognition of SAG1 by sera from infected patients. The 4F11E12 Fab fragment was created by digestion with papain

Origin Pub PMID: 16242717

Buffer Composition: PBS only.

Uniprot Accession No.: C7E5U4

Specificity Statement: 4F11E12 recognises a highly conserved conformational epitope on the surface of the D1 domain in both native and recombinant SAG1, located within the N-terminal domain; the binding site does not overlap with the proposed ligand-binding pocket or homodimerization site. The eptiope is an immunodominant area and includes residues from 2 long loops located at one exteremity. SAG1 is a GPI-anchored cell surface protein predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. SAG1 recognizes ligands such as glucosamide moieties, which are expressed in a broad range of host cell types. T. gondii is a protozoan parasite capable of causing chronic infections resulting in congenital disease and has the ability to evade natural host defences.

Application Notes (Clone): F411E12 can be co-crystallized with SAG1, which enables the mode of binding to be elucidated.

• Artikelnummer: ABAAb00763-15.0-BT
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00763-15.0-BT
• Verpackungseinheit: 500 μg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Recombinant
• Isotyp: IgM kappa
• Wirt: Human