Anti-single-stranded poly(rI) RNA (Jel 103)

Anti-single-stranded poly(rI) RNA [Jel 103], Recombinant, IgG kappa, Rabbit

CloneID: Jel 103

Antigen Long Description: Jel 103 was selected from a hybridoma cell line generated by the fusion of the MOPC 315-43 myeloma cell line with mouse spleen cells derived from C57/B1 mice which had been immunized with poly(rI)poly(dC) duplex complexed to methylated bovine serum albumin.

Origin Pub PMID: 7523684

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Specificity Statement: Jel 103 is highly specific for single-stranded RNA and shows sequence specificity as it binds to poly(rI) but not to poly(dI), poly(rG) or to any other homopolymer - it does not bind randomly to calf thymus DNA. Jel 103 Fab is shown to form base stacking interactions with a Tyr residue and two salt-bridge interactions to the phosphate backbone with Lys and Arg residues.

Application Notes (Clone): Jel 301 can be used for the detection of single-stranded poly(rI) and for binding studies where the antibody has been used previosly in solid-phase radioimmunoassays.

• Artikelnummer: ABAAb00800-23.0
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab00800-23.0
• Verpackungseinheit: 200 μg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Recombinant
• Methode: Immunofluorescence
• Isotyp: IgG kappa
• Wirt: Rabbit