CloneID: AbAb1-pSTAT3
Antigen Long Description: The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pSTAT3 (PGSAAP-pY-LKTKGGGSC) conjugated with KLH as an immunogen.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P40763
Specificity Statement: This antibody binds human phosphorylated STAT3. This protein mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors.
Application Notes (Clone): The binding reactivity of this antibody to recombinant phosphorylated pSTAT3 peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of STAT3 protein IFN-α-treated cells. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pSTAT3 in the cell lysate of HeLa cells treated with IFN-α (US20090233358).
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