CloneID: 3F9
Antigen Long Description: The original antibody was generated by immunizing a male Bactrian camel with TeA-CMO-KLH followed by antibody library construction and phage display-based selection.
Origin Pub PMID: 32702970
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for tenuazonic acid, an Alternaria mycotoxin. The antibody does not cross-react with AOH, AME, DON, ITeA, or ZEN.
Application Notes (Clone): The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6×His tag secondary antibody. The IC50 value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.
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