CloneID: scFvTc
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with tetracycline hydrochloride-BSA as an immunogen. The cDNA obtained from total mRNA of the immunized mouse splenocytes was used as a template to amplify the variable domain genes and generate an scFv phage display library. Later on the antibody was selected by panning this library against tetracycline-ovalbumin conjugate.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody binds tetracycline. Tetracycline, is an antibiotic derived from microorganisms of the genus Streptomyces and are broadly used to treat bacterial infections. This antibody is reported to cross-react with aureomycin and also shows weak reactivity for oxytetracycline and doxycycline.
Application Notes (Clone): The binding characterization of this antibody for tetracycline was done using ELISA. Additionally, this antibody was also capable of cross-reacting with aureomycin, oxytetracycline and doxycycline in an ELISA. The colloidal gold labelled version of this antibody was used for the development of colloidal gold test strip for detection of tetracycline hydrochloride (CN103396484).
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