Anti-toxin A (A20.1)

Anti-toxin A [A20.1], Recombinant, VHH, Camelid

CloneID: A20.1

Heavy Chain modification: His-tagged

Antigen Long Description: The original antibody was generated by immunizing a llama with recombinant RBD fragments TcdA-RBD-f1 and TcdB-RBD-f1, followed by phage display library construction and panning against TcdA-RBD-f1.

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P16154

Specificity Statement: The antibody is specific for toxin A of Clostridium difficile. It recognises a conformational epitope on native C. difficile toxin.

Application Notes (Clone): The specificity of the original format of the antibody to TcdA was confirmed by ELISA analysis. The binding affinity of the original format of the antibody was measured by surface plasmon resonance (Kd= 2 nM). Human lung fibroblast (HLF) cytotoxicity assay was used to determine whether the original format of the antibody could neutralize TcdA-induced HLF cell rounding. The antibody showed neutralizing activity to TcdA (US9771416B2).

• Artikelnummer: ABAAb04200-34.11
• Hersteller: Absolute Antibody
• Hersteller Artikelnummer: Ab04200-34.11
• Verpackungseinheit: 100 μg
• Mengeneinheit: STK
• Reaktivität: Various species
• Klonalität: Recombinant
• Methode: ELISA, Neutralization, Super-Resolution Microscopy, BLI Binding Assay
• Isotyp: VHH
• Wirt: Camelid