CloneID: HpU-2
Antigen Long Description: HpU-2 was prepared by subcutaneous injection of purified H. pylori urease into BALB/c mice.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P14916
Specificity Statement: HpU-2 binds specifically to H. pylori at the epitope with sequence SVELIDIGGNRRIFGFNALVDR - the sequence is part of a loop near to the C-terminus of the alpha subunit of urease (the beta subunit possesses the active site). HpU-18 competes with HpU-2 for binding to this epitope. H. pylori is a gram negative spiral bacterium and causes numerous gastroduodenal diseases. It expresses large amounts of urease when present in the human stomach; urease is key for the survival and pathogenesis of the bacteria as it neutralizes the acidity in the stomach by hydrolysis of urea into ammonia.
Application Notes (Clone): HpU-2 results in ~82% inhibition (allosteric inhibition) of urease activity and the inhibitory activity is increased to 87% when HpU-2 is used in combination with HpU-19. HpU-2 has been shown be unsuccessful for use in IHC of formalin-fixed sections of H. pylori-infected human cells. HpU-2 can also be used in SPR and ELISA studies.