CloneID: A9
Heavy Chain modification: ScFv
Antigen Long Description: A DIII‐immune phage display scFv library was generated by immunizing three chickens against group A and group I USUV DIII. The original antibody was isolated from this library by panning against Usutu virus A and Usutu virus B.
Origin Pub PMID: 32829514
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted chicken scFv antibody, based on the original chicken scFv format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q5WPU5; Q9Q6P4
Specificity Statement: This antibody binds an epitope within domain III of the envelope protein of Usutu virus (USUV). Usutu virus is a mosquito-borne flavivirus belonging to the Japanese encephalitis virus (JEV) serocomplex, which is an emerging zoonotic arbovirus of concern because of its pathogenicity to humans. This virus propagates via a zoonotic life cycle with birds as amplifying hosts and ornithophilic mosquitoes acting as vectors. USUV infections cause mild influenza‐like symptoms, in sever cases invasion of the central nervous system in immunocompromised patients causing meningoencephalitis and meningitis.
Application Notes (Clone): This antibody can bind the E protein domain III antigens of USUV strains A, B, I and West Nile virus in an ELISA. Surface plasmon resonance was used for the determination of binding affinity of this scFv antibody. The scFv antibody binds USUS I and WNV domain III with a Kd of 0.29–0.38 μM and 0.10 μM respectively. This antibody can also detect USUV E protein DIII from groups A, B, and I as well as WNV 2741 E protein DIII in a western blot (PMID: 32829514).
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