Anti-Vimentin (MO82)

Anti-Vimentin [MO82], Recombinant, IgG1 kappa, Rat
Artikelnummer
ABAAb03912-6.1
Verpackungseinheit
100 μg
Hersteller
Absolute Antibody

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CloneID: MO82

Antigen Long Description: The original antibody was generated by immunizing BDF1 mice with keyhole limpet hemocyanin (KLH) coupled PV82 — a synthesized vimentin peptide containing the ser82 phosphorylation — (PV82-KLH) emulsified in complete Freund's adjuvant.

Origin Pub PMID: 8605039

Buffer Composition: PBS with 0.02% Proclin 300.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: P31000

Specificity Statement: This antibody specifically recognizes vimentin phosphorylation at Ser82, a CAM kinase II phosphorylation site.

Application Notes (Clone): This antibody is specific to the CAM kinase II phosphorylated vimentin Ser82 residue, as determined by ELISA, can detect the activity of endogenous CAM kinase II astrocytes, as detected by WB, and was used for ICC (IF) to detect CAM kinase II phosphorylated vimentin Ser82 in type 1 astrocytes extracted from newborn rats' cerebral cortices. IEM observation confirmed that the epitope of MO82 was localized along the vimentin filaments (Ogawara et al., 1995; PMID: 7490282). This antibody was used for: in vivo vimentin kinase activity visualization of vimentin kinase activity in cell signaling or mitosis (Goto et al., 1998; PMID: 9565595) and as a positive control in a WB for vimentin ser82 phosphorylation and in IF staining of U251 human glioma cells (Yamaguchi et al., 2005; PMID: 16260496).
Mehr Informationen
Artikelnummer ABAAb03912-6.1
Hersteller Absolute Antibody
Hersteller Artikelnummer Ab03912-6.1
Verpackungseinheit 100 μg
Mengeneinheit STK
Reaktivität Human, Mouse (Murine), Rat (Rattus)
Klonalität Recombinant
Methode Immunofluorescence, Western Blotting, ELISA, Immunocytochemistry, Electron microscopy (EM)
Isotyp IgG1 kappa
Wirt Rat
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