CloneID: A1
Antigen Long Description: The original antibody was generated by immunizing mice with WSSV followed by antibody library construction and phage display-based selection.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody is specific for WSSV. The antibody binds to native WSSV, and not to the denatured WSSV.
Application Notes (Clone): The binding specificity of the scFv antibody was determined by ELISA (Dai et al., 2003; PMID: 12969566 and Yuan et al., 2006; PMID: 17140131). The antibody had an affinity constant (Kaff) of 2.02 x 10^9 M-1. Dot blot assays showed that the antibody could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV (Dai et al., 2003; PMID: 12969566).
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