CloneID: M55
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing SCID BALB/c mice with vesicular stomatitis virus (VSV).
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P0C2X0
Specificity Statement: This antibody reacts with a defined subsite within the major antigenic site of VSV-G. The exact epitope is not defined.
Application Notes (Clone): This stable, recombinant single-chain Fv-Cκ (scFv-Cκ) antibody fragment was generated by protoplast fusion of J558L myeloma cells with Escherichia coli K803 transformed with an appropriate scFv-Cκ expression construct. The aptly named M55 scFv-Cκ expression construct — containing a CDR2 Ser55-to-Arg substitution (T-to-G transition at the third base), found in the VH chain of several hypermutated antibodies (VI22, VI43, and VI53) — was derived from the secondary antibody clone VI24. M55 binds a defined subsite within the major antigenic site of VSV-G. However, the exact binding site in the subunit is not defined. M55 binding to VSV-G was measured using solid-phase ELISA. The VH Ser55-to-Arg substitution in M55, which is not found in its parental clone VI24, increased monovalent binding by about 10-fold compared with M0 (the germline derived scFv-Cκ). Furthermore, crosslinking with HRP labeled mouse anti-Cκ antibodies improved M55 binding 10-fold (Kalinke et al., 2000; PMID: 10963674).
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