Aurora A Protein Kinase, His-Tag (HEK293-derived) Recombinant

Products from BPS Bioscience require a minimum order value above 400€

Encompassing Amino Acids: 2-403 (end)

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Assay was done in Kinase buffer containing 2 mM DTT using KEMPtide substrate (0.2 mg/ml) and 10 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent.

Description: Human Aurora kinase A also known as, AURKA, Serine/Threonine-Protein Kinase Aurora-A, and Breast Tumor-Amplified Kinase, GenBank Accession No. NM_003600, a.a. 2-403 (end) with N-terminal His-tag, expressed in a HEK 293 cell expression system. MW =46 kDa.

Format: Aqueous solution

Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 200mM Imidazole, 20% glycerol, and 3 mM DTT.

Genbank: NM_003600

Storage Stability: At least 6 months at -80°C.

Supplied As: Aqueous buffer solution.

Tags: N-terminal His-tag

Uniprot: O14965

Warnings: Avoid multiple freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Ouchi, M. et al., J. Biol Chem. 279 (19),19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5(6), 853-864 (2003).

• Artikelnummer: BPS100112
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 100112
• Green Labware: Nein
• Verpackungseinheit: 10 µg
• Mengeneinheit: STK