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Application: Expression of human B2M HLA-A*01:01 in cells of interest.Generate B2M HLA-A*01:01 expressing cell pools or stable cell lines by puromycin selection.
Background: Human Leukocyte Antigen-A (HLA-A) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-A and β2-microglobulin (B2M). There are over 200 genes encoding HLA variants and this variability plays a critical role in adaptive immunity. HLA-A*01:01 is more common in Europe. It has been broadly linked to autoimmune disorders, type 1 diabetes, and infectious diseases. The presence of this haplotype was considered a risk for the development of severe COVID19, while it was also reported that it had a protective effect against severe bilaterial pneumonia. Studies in third wave patients pointed to an effect in forming long-term T cell immunity. Further in-depth studies are needed to fully understand the role of this haplotype as allele of risk or protection in immune-related disorders.
Description: B2M HLA-A*01:01 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses result in the expression of B2M (beta-2 microglobulin) HLA (human leukocyte antigen)-A*01:01 driven by an EF1a promoter. The lentiviruses also contain a puromycin selection marker (Figure 1).Figure 1. Schematic of the lenti-vector used to generate B2M HLA-A*01:01 Lentivirus.
Formulation: The lentivirus particles were produced in HEK293T cells. They are supplied in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations and produced at higher titers by special request, for an additional fee.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Target: B2M, HLA
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
SIN Vector?: Yes
References: Gornalusse G., et al., 2017 Nature Biotechnology 35:765-772.Shkurnikov M., et al., 2023 PeerJ 11:e14707.
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