Shelf life (days): 1460.0
Formulation: An oil
Formal Name: 3',6'-bis[(acetyloxy)methoxy]-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester
Purity: ≥95%
Formula Markup: C42H40O21
Formula Weight: 880.7
Notes: BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH.{25713,25712} This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours.{25710} Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm.{25712,25711} This approach is amenable to either spectrofluorometry or flow cytometry.{25712,25709} BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.{25708}
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