Formal Name: 5-bromo-4-chloro-1H-indol-3-ol 3-(dihydrogen phosphate) compd. with 4-methylbenzenamine
Purity: ≥95%
Formula Markup: C8H6BrClNO4P / C7H9N
Formula Weight: 433.621141
CAS Number: 6578-06-9
Notes: BCIP is a colorimetric substrate for alkaline phosphatase.{60622,60623} Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).