BCL-2, His-Tag Recombinant

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Encompassing Amino Acids: 1-211

Applications: Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.

Assay Conditions: Following theprotocol for BPS Cat. #50222. Briefly,BCL-2 was combined with BCL-2peptide ligand in TR-FRET assay bufferalong with anti-His Tb donor and dyelabeledacceptor. Varying concentrationsof ABT 199 were added and the reactionwas incubated at room temperature for 2hours followed by fluorescent intensitymeasurements.

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Description: Human Bcl-2 (GenBank Accession No. P10415), a.a. 1-211 with C-terminal His-tag, MW = 27 kDa, expressed in an E. coli expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and variable imidazole

Genbank: P10415

Host Cell Line: E. coli

Purity: ≥90%

Storage Stability: At least 6 months at -80°C.

Tags: C-terminal His-tag

Uniprot: P10415

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Labi V. et al., Cell Death Differ. 2008 Jun;15(6):977-87.
2. Rong Y.P. et al., Mol Cell. 2008 Jul 25;31(2):255-65.

• Artikelnummer: BPS50272
• Hersteller: BPS Bioscience
• Hersteller Artikelnummer: 50272
• Green Labware: Nein
• Verpackungseinheit: 100 µg
• Mengeneinheit: STK