Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820), CF740 conjugate, 0.1mg/mL

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820), CF740 conjugate, 0.1mg/mL
Artikelnummer
BTMBNC741897-500
Verpackungseinheit
500 uL
Hersteller
Biotium

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Description: Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: rCALD1/820

Immunogen: Recombinant full-length human CALD1 protein

Antibody Reactivity: Caldesmon, HMW

Entrez Gene ID: 800

Z-Antibody Applications: IHC, FFPE (verified)/WB (verified)

Verified AB Applications: IHC (FFPE) (verified)/WB (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Does not react with rat/Immunohistology (formalin): 0.25-0.5 ug/mL for 30 minutes at RT/Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 7.5-8.5 for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
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Artikelnummer BTMBNC741897-500
Hersteller Biotium
Hersteller Artikelnummer BNC741897-500
Verpackungseinheit 500 uL
Mengeneinheit STK
Reaktivität Human
Klonalität Recombinant
Methode Western Blotting, Immunohistochemistry
Isotyp IgG1 kappa
Wirt Mouse
Konjugat Conjugated, CF740
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