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Encompassing Amino Acids: 39-426
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to the protocol in CBL-B TR-FRET Assay Kit (BPS Bioscience #79575).
Description: Recombinant human CBLB (Cbl proto-oncogene B), encompassing amino acids 39-426. This construct contains an N-terminal GST-tag followed by a PreScission Protease Cleavage site to facilitate removal of the GST portion and contains the mutation of interest (Y363E). The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, and variable glutathione
Genbank: NM_170662
Purity: ≥90%
Tags: N-terminal GST-tag
Uniprot: Q13191
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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