Description: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: ICO-115
Immunogen: Blast cells of a chronic myeloid leukemia patient
Antibody Reactivity: CD34
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Biol Regulators Homeostatic Agents (2001) 15(1):1-13. (IHC, FFPE; IHC, frozen; WB)
- Am J Physiol (2004) 287(6), H2670-H2676. (Flow)
Entrez Gene ID: 947
Expected AB Applications: IF/Flow, surface (published for clone)/IHC (FFPE) (published for clone)/IHC (frozen) (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow (verified)/Flow, surface (published)/IF/IHC, FFPE (published)/IHC, frozen (published)/WB (published)
Verified AB Applications: Flow (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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