Description: Reacts with five invariable CD3 chains designated CD3y, CD3d, CD3e, CD3f, and CD3n with molecular weight ranging from 16-28 kDa. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: RIV9
Immunogen: Human peripheral lymphocytes
Antibody Reactivity: CD3
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Transplant Proc (1989) 21: 1026-7. (functional studies)
- Eur J Immunol (1990) 20(4): 943-945. (immunoprecipitation)
- Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989.
- Immunology (1994) 83: 457-464. (immunofluorescence, frozen tissue)
- Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995.
Entrez Gene ID: 916
Expected AB Applications: IHC (frozen)/Functional studies (published for clone)/IP (published for clone)
Z-Antibody Applications: Functional studies (published)/IHC, frozen (expected)/Flow, surface (verified)/IF (verified)/IP (published)
Verified AB Applications: Flow (surface) (verified)/IF (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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