Description: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes, and is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and CD3 is predominantly intracytoplasmic in cortical thymus.Anti-CD3 clone OKT3 has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. In the crystal structure of the antibody-receptor complex, clone OKT3 was found to bind a conformational epitope on CD3e dominated by a linear stretch in the F-G loop of the subunit.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: OKT3
Immunogen: Human PBMCs
Antibody Reactivity: CD3e
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Cell Biol (1992) 267(17): 11685-11688. (immunoprecipitation)
- PNAS USA (2004) 101(20): 7675-7680. (crystal structure of antibody/antigen complex)
- J Immunol (2005) 174:3773-3780. (IHC, frozen)
- Blood (2011) 117: 5463-5472. (in vivo cell depletion)
- J Immunol (2012) 188:5538-5546. (functional studies)
Entrez Gene ID: 916
Expected AB Applications: IHC (frozen) (published for clone)/Functional studies (published for clone)/IP (published for clone)
Z-Antibody Applications: Functional studies (published)/IHC, frozen (published)/Flow, surface (verified)/IF (verified)/IP (published)
Verified AB Applications: Flow (surface) (verified)/IF (verified)
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