Description: This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: B6H12.2
Immunogen: Intact CD47 purified from placenta
Antibody Reactivity: CD47
References: Note: References for this clone sold by other suppliers may be listed for expected applications.1. J Biol Chem (2002) 277(12): 10028-10036. (functional studies)2. Cell (2009) 138(2): 286-299. (functional studies)3. J Immunol (2014) 193: 3914-3924. (immunofluorescence)4. J Immunol (2015) 195: 661-671. (flow, surface; immunofluorescence; western)5. Nat Med (2019) 25(4): 612-619. (immunoprecipitation)
Entrez Gene ID: 961
Expected AB Applications: Functional studies (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: IF (published)/Flow, surface (verified)/Functional studies (published)/IF (verified)/IP (published)/WB (published)
Verified AB Applications: Flow (surface) (verified)/IF (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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