CD5(Cris-1), CF740 conjugate, 0.1mg/mL

Description: Recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: Cris-1

Immunogen: Stimulated human leukocytes

Antibody Reactivity: CD5

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. Immunology (1990) 70, 434-439. (Flow)
2. J Immunol (1992) 148(5):1287-93. (receptor activation)
3. J Immunol (1993) 151 (9): 4423-4430. (IP)
4. Nature (1991) 351: 662-665. (ELISA)

Entrez Gene ID: 921

Expected AB Applications: ELISA (published for clone)/Functional studies (published for clone)/IP (published for clone)

Z-Antibody Applications: Flow (verified)/ELISA (published)/Functional studies (published)/IP (published)

Verified AB Applications: Flow (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Immunofluorescence 0.5-1 ug/mL Immunoprecipitation 0.5-1 ug/500 ug protein lysate/Optimal dilution for a specific application should be determined by user

• Artikelnummer: BTMBNC740176-500
• Hersteller: Biotium
• Hersteller Artikelnummer: BNC740176-500
• Verpackungseinheit: 500 uL
• Mengeneinheit: STK
• Reaktivität: Human, Rhesus Monkey (Primate)
• Klonalität: Monoclonal
• Methode: Immunoprecipitation, ELISA, Flow Cytometry, Functional Studies
• Isotyp: IgG2a kappa
• Wirt: Mouse
• Konjugat: Conjugated, CF740