Description: Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: LAM1-116
Immunogen: MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.
Antibody Reactivity: CD62L/L-Selectin
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Clin Invest (1997) 99(8): 2036-2044. (Flow)
- J Clin Invest (1997) 100(5): 996-1005. (functional studies)
- J Biol Chem (1997) 272(21): 13961-13965. (Flow, IP)
- Ann Rheum Dis (2000) 59:297-299. (ELISA)
- J Immunol (2002) 169:2034-2043. (ELISA)
- Immunity (2003) 19(5): 713-724. (WB)
- Fert Stert (2007) 88(4) Supplement: 1207-1211. (IHC, FFPE)
- J Leukocyte Biol (2008) 83: 99-105. (Flow, IF)
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Entrez Gene ID: 6402
Expected AB Applications: ELISA (published for clone)/Flow, surface (published for clone)/Functional studies (published for clone)/IHC (FFPE) (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IHC, FFPE (published)/ELISA (published)/IP (published)/WB (published)
Antibody Application Notes: ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Western blot 0.5-1.0 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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