Description: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: AN4.3
Immunogen: Recombinant full-length human Cdk2 protein
Antibody Reactivity: Cdk2/p34cdc2 Serine-Threonine Kinase
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
Cell Cycle (2016) 15(23):3203-3209. (WB)
Entrez Gene ID: 1017
Expected AB Applications: WB (published for clone)
Z-Antibody Applications: WB (published)
Antibody Application Notes: Inhibits activation of p34cdc2 kinase by cyclins; Immunoprecipitation; Kinase Assay; Western blot; Flow cytometry: 1-2 ug/mL; Optimal dilution for a specific application should be determined.
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