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Chemi-Verse™ MEK1 Kinase Assay Kit
Chemi-Verse™ MEK1 Kinase Assay Kit
Artikelnummer
BPS82100
Verpackungseinheit
96 reactions
Hersteller
BPS Bioscience
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Products from BPS Bioscience require a minimum order value above 400€
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: MEK1, also known as dual specificity mitogen-activated protein kinase kinase 1 or MAP2K1, is part of dual-specificity protein kinase family. MEK1 activates ERKs (extracellular signal-regulated kinases or MAPs) in response to several extra- and intracellular signals, and it is thus involved in cell proliferation and differentiation via regulation of transcription. MEK1 can be seen as the gatekeeper of ERK1/2 activity, offering a unique opportunity for inhibitors to act on all the downstream members of the ERK pathway. MEK1/2 has a very unique hydrophobic pocket which allows for binding of non-ATP competitive allosteric inhibitors, disturbing the formation of RAF (proto-oncogene serine/threonine-protein kinase)-MEK complexes or MEK phosphorylation. Inhibitors for MEK1 are currently used in combination with B-RAF1 inhibitors for the treatment of B-RAF-mutant melanoma. Further studies are required to design inhibitors that act mainly in abnormal tissues, as MEK1 is also expressed in normal cells, and to bypass resistance mechanisms.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ MEK1 Kinase Assay Kit is a luminescent assay designed to measure MEK1 (dual specificity mitogen-activated protein kinase kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant MEK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Caunt C., et al., 2015 Nature Reviews Cancer 15: 577-592.
Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: MEK1, also known as dual specificity mitogen-activated protein kinase kinase 1 or MAP2K1, is part of dual-specificity protein kinase family. MEK1 activates ERKs (extracellular signal-regulated kinases or MAPs) in response to several extra- and intracellular signals, and it is thus involved in cell proliferation and differentiation via regulation of transcription. MEK1 can be seen as the gatekeeper of ERK1/2 activity, offering a unique opportunity for inhibitors to act on all the downstream members of the ERK pathway. MEK1/2 has a very unique hydrophobic pocket which allows for binding of non-ATP competitive allosteric inhibitors, disturbing the formation of RAF (proto-oncogene serine/threonine-protein kinase)-MEK complexes or MEK phosphorylation. Inhibitors for MEK1 are currently used in combination with B-RAF1 inhibitors for the treatment of B-RAF-mutant melanoma. Further studies are required to design inhibitors that act mainly in abnormal tissues, as MEK1 is also expressed in normal cells, and to bypass resistance mechanisms.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Chemi-Verse™ MEK1 Kinase Assay Kit is a luminescent assay designed to measure MEK1 (dual specificity mitogen-activated protein kinase kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant MEK1 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Caunt C., et al., 2015 Nature Reviews Cancer 15: 577-592.
| Artikelnummer | BPS82100 |
|---|---|
| Hersteller | BPS Bioscience |
| Hersteller Artikelnummer | 82100 |
| Green Labware | Nein |
| Verpackungseinheit | 96 reactions |
| Mengeneinheit | PAK |
| Produktinformation (PDF) | Download |
| MSDS (PDF) |
|
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