Description: This Mab recognizes Mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a and the CD103 DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31( ) dendritic cells and on a small subset of CD14( ) and CD16(-) monocytes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Rattus norvegicus (laboratory rat), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: 1F6
Immunogen: RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.
Antibody Reactivity: CLEC9A
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Nature (2009) 458(7240): 899-903. (functional studies)
- J Immunol (2015) 195:5066-5076. (Flow, surface)
Entrez Gene ID: 232414
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)
Antibody Application Notes: Flow cytometry: 0.5-1 ug/million cells; Western blot 0.5-1 ug/mL; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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