CLEC9A / DNGR1 (Dendritic Cell Marker)(8F9), CF740 conjugate, 0.1mg/mL

Description: This MAb recognizes a protein of 27 kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: 8F9

Immunogen: RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope

Antibody Reactivity: CLEC9A/DNGR1

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. J Clin Invest (2008) 118(6):2098-2110. (IF, Flow)
2. Clinical Translational Immunol (2016) 5:e57. (Flow)

Entrez Gene ID: 283420

Expected AB Applications: Flow, surface (published for clone)/IF (published for clone)

Z-Antibody Applications: Flow, surface (published)/IF (published)

Antibody Application Notes: ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• Artikelnummer: BTMBNC743063-100
• Hersteller: Biotium
• Hersteller Artikelnummer: BNC743063-100
• Verpackungseinheit: 100 uL
• Mengeneinheit: STK
• Reaktivität: Human
• Klonalität: Monoclonal
• Methode: Immunofluorescence, Flow Cytometry
• Isotyp: IgG2a kappa
• Wirt: Mouse
• Konjugat: Conjugated, CF740