Target Synonym: CNOT1;AD-005;CDC39;NOT1;NOT1H
Storage: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Background: Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES cell identity.
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Immunogen: Recombinant fusion protein of human CNOT1
Dilution: WB 1:200-1:2000;IF 1:50-1:200
Calculated MW: 173kDa/241kDa/266kDa
Observed MW: 267kDa
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