Shelf life (days): 1460.0
Formulation: A crystalline solid
Formal Name: 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one
Purity: ≥95%
Formula Markup: C26H21N3O
Formula Weight: 391.5
CAS Number: 70217-82-2
Notes: Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Item No. 16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.{26296} Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.{26291,26296} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}
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