Description: This antibody recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alphalpha-helicaldomains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Rattus norvegicus (laboratory rat), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: R5.21C
Immunogen: Mouse liver DOC-JR-plasma membranes
Antibody Reactivity: Connexin-32
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Cell Biol (1986) 103 (3): 755-766. (WB)
- Endocrinology (1993) 133: 2371-2378. (IF)
- J Cell Sci (1994) 107: 955-967. (IP)
- Int J Cancer (1997) 73: 479-485. (IHC, frozen)
Entrez Gene ID: 14618 (Mouse)
Expected AB Applications: IHC (frozen) (published for clone)/IF (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: IF (published)/IHC, frozen (published)/IP (published)/WB (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Flow cytometry: 0.5-1 ug/million cells/Immunohistology (frozen) 0.5-1 ug/mL/Western blotting 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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