Description: Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene. The CYP2 family are part of the microsomal drug metabolising system that is responsible for oxidation of many therapeutic agents as well as steroids, fatty acids and many other endogenous substances. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. P6 is highly specific for members of the CYP 3A gene family and cross reacts with CY 3A4 in human, CYP 3A in rat and potentially CYP 3A11 (based on inducibility) in mice.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: P6
Immunogen: Full length protein (Rat). Rat liver cytochrome P450 3A1.
Antibody Reactivity: CYP3A1/Cytochrome P450 3A1
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Eur J Biochem (1993) 213: 805-814. (cell ELISA)
- Toxicol in Vitro (1994) 8(3): 423-35. (ELISA)
- Drug Metab Disp (2001) 29(3) Pt 2: 353-360. (WB)
Entrez Gene ID: Not Known
Expected AB Applications: ELISA (published for clone)/WB (published for clone)
Z-Antibody Applications: ELISA (published)/WB (published)
Antibody Application Notes: ELISA: For coating, order antibody without BSA; Flow cytometry: 0.5-1 ug/million cells; Western blot 0.5-1.0 ug/mL; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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