Description: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: C11
Immunogen: Keratin-enriched preparation from cultured human A431
Antibody Reactivity: Cytokeratin, multi
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
Clin Cancer Res (2012) 18(4): 993-1003. (IHC, FFPE; western)
Entrez Gene ID: 3851 (CK4); 3852 (CK5); 3853 (CK6A); 3854 (CK6B); 286887 (CK6C); 3856 (CK8); 3858 (CK10); 3860 (CK13); 3875 (CK18)
Expected AB Applications: WB (published for clone)
Z-Antibody Applications: Flow, intracellular (verified)/IF (verified)/IHC, FFPE (verified)/WB (published)
Verified AB Applications: Flow (intracellular) (verified)/IF (verified)/IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistology formalin-fixed 0.5-1.0ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Immunofluorescence 0.5-1.0 ug/mL/Western blotting 0.5-1.0 ug/mL/Optimal dilution for a specific application should be determined by user
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