iLite® FGF-21, Assay Ready Cells

The iLite® FGF21 Assay Ready Cells can be used in an assay for the quantification of the potency of analogues of human FGF21, the potency of FGFR1c antagonists or a neutralizing antibody response against such products in human serum in the absence of serum matrix effects. Human fibroblast growth factor 21 (FGF21) is a member of a family of the atypical fibroblast growth factors that include FGF19 and FGF23 in man. FGF21 lacks the heparin-binding domain of conventional FGFs and can consequently diffuse throughout the body and function as a hormone. FGF21 stimulates glucose uptake in adipocytes and the effects on glucose uptake is additive with insulin. The iLite® FGF21 Assay Ready Cells are a genetically engineered reporter gene cell line responsive to FGF21 by specific and proportional expression of Firefly Luciferase. The receptor chain FGFR1c is overexpressed on the surface together with the co-factor β-Klotho which has been genetically optimized to enhance sensitivity. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

• Artikelnummer: WIEBM3071
• Hersteller: Svar Life Science / Eurodiagnostica
• Hersteller Artikelnummer: BM3071
• Verpackungseinheit: 250 µL
• Mengeneinheit: PAK