Description: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Bloom's syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: 1A9
Immunogen: Full-length native cow LIG1 protein
Antibody Reactivity: DNA Ligase 1
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- PNAS USA (1987) 84:1374-1378. (Flow, IP)
- Eur J Immunol (1990) 20: 509-515. (functional studies)
- J Immunol (2003) 171:2496-2503. (Flow; IP; functional studies)
Entrez Gene ID: 3978
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IP (published)
Antibody Application Notes: Western blot 0.5-1 ug/mL; ELISA; Immunoprecipitation; Optimal dilution for a specific application should be determined.
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