Shelf life (days): 730.0
Assay Range: 1.17-150 ng/ml
Sensitivity: 80% B/B0: ~3 ng/ml
Cross Reactivity: 5-Methyl-2'-deoxycytidine 100%; 5-Methylcytidine 20%; 2-Deoxycytidine 0.1%; Cytidine 0.1%; Thymidine <0.01%
Formula Weight: 0.0
Notes: DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2-deoxy cytidine yielding the modified base 5-methyl-2-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division. Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Cayman’s DNA Methylation ELISA is a competitive assay that can be used for quantification of 5-methyl-2-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices. The ELISA typically displays IC50 (50% B/B0) and IC80 (B/B0) values of approximately 12 and 3 ng/ml, respectively.
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